Determination of Dihydrostreptomycin Sulfate in Milk by HPLC Using Ion-Pair and Postcolumn Derivatization
- 1 July 1995
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 18 (13) , 2695-2702
- https://doi.org/10.1080/10826079508009319
Abstract
An isocratic, paired-ion reversed-phase high-performance liquid chromatographic method for the determination of dihydrostreptomycin sulfate in milk by postcolumn derivatization has been developed. The milk was treated with 85% trichloroacetic acid to precipitate proteins, and the supernatant clean-up was performed using a tC18 pretreatment column. The correlation coefficients were 0.999. The lower limit of quantification was 25 ng/ml and the limit of detection close to 15 ng/ml. The recovery of dihydrostreptomycin sulfate varying from 82.6 to 82.8%.Keywords
This publication has 5 references indexed in Scilit:
- Fluorescence HPLC determination of streptomycin in meat using ninhydrin as a postcolumn labeling agent.BUNSEKI KAGAKU, 1988
- Post-column derivatization system for the fluorimetric determination of guanidino compounds with ninhydrin by reversed-phase ion-pair high-performance liquid chromatographyJournal of Chromatography A, 1987
- Fluorometric determination of streptomycin in serum by high-performance liquid chromatography using mobile phase containing fluorogenic reagentAnalytical Biochemistry, 1987
- PRESENCE OF DIHYDROSTREPTOMYCIN AND PENICILLIN IN COWS' MILK FOLLOWING INTRAUTERINE ADMINISTRATIONAustralian Veterinary Journal, 1982
- STREPTOMYCES ANTIBIOTICS. IX. DIHYDROSTREPTOMYCINJournal of the American Chemical Society, 1946