The microbiological assay of riboflavin. The influence of inorganic constituents and unknown growth factors

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Abstract
For Lactobacillus casei e, optimum mineral concs. per 1. of basal medium were found to be: 0.4 mg. FeSO4 7H2O, Ca++, 31-35 mg., KH2PO4, 2.25 g. and NaCl 4.65 g. Increases in Fe++, K-, POr and NaCl did not greatly decrease acid production, but addition of Ca++ did so. The necessity for, and effect of, the addition of a liver eluate to the basal medium were shown. The importance of these results in the assay of foodstuffs for riboflavin was discussed.

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