In vitro translation of cytosolic and peroxisomal epoxide hydrolase and catalase on liver polyribosomes from untreated and clofibrate‐treated C57B1/6 mice
- 22 May 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (1) , 83-88
- https://doi.org/10.1016/0014-5793(89)80021-3
Abstract
Free and membrane‐bound polyribosomes were isolated from the livers of untreated and clofibrate‐treated male C57B1/6 mice. The in vitro translation products were investigated in a rabbit reticulocyte cell‐free system by immunoprecipitation of cytosolic epoxide hydrolase, catalase and albumin. The soluble forms of epoxide hydrolase present in cytosol and in peroxisomes were found to be synthesized on free polyribosomes and could not be distinguished from each other, since only one primary translation product was found with the methods used. Clofibrate treatment was found to increase total protein synthesis, synthesis of soluble epoxide hydrolase and translational efficiency of the isolated polyribosomes.Keywords
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