Thymidine Kinase Activity and its Relationship to DNA Synthesis in the Primary Root of Vicia faba, L.

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Abstract
The enzyme thymidine kinase can be induced, or activated, in the apical 6 cm of the primary root of Vicia faba. The activity of this enzyme is correlated with the amount of thymidine triphosphate present in successive 6-mm segments of the root. Thymidine is produced by cell death. Some of this thymidine may reach the meristem and be used by the apical cells in DNA synthesis, once it is phosphorylated by thymidine kinase. Most remains in the basal region of the root, however, where it is used by differentiating cells. Non-radioactive thymidine, at the concentrations used, has no effect on root growth by elongation.

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