GABA‐like immunoreactivity in the cat retina: Light microscopy

Publisher Website
Google Scholar
Abstract
Semithin sections of the cat retina were stained with antibodies against GABA conjugated to bovine serum albumin with glutaraldehyde. Labelled cells were visualized by means of the peroxidase‐antiperoxidase method. In the outer plexiform layer both A‐ and B‐type horizontal cells and the B‐type axon terminal system expressed GABA‐like immunoreactivity. Approximately 25–30% of all amacrine cells and the whole inner plexiform layer were heavily labelled. Two types of putative GABA‐ergic interplexiform cells could be distinguished. One of them also expressed tyrosine‐hydroxylase‐like immunoreactivity. A few bipolar cells were also GABA‐immunolabelled. GABA‐like immunoreactivity and 3H‐muscimol uptake were colocalized in 90% of the amacrine cells labelled. However, horizontal cells did not accumulate 3H‐muscimol.

This publication has 72 references indexed in Scilit: