Production of L-DOPA from cell suspension culture of Mucuna pruriens f. pruriens
- 1 June 1994
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 13 (9) , 519-522
- https://doi.org/10.1007/bf00232948
Abstract
Production of L-DOPA was studied in cell suspension culture of Mucuna pruriens f. pruriens. Suspension culture was established in MSI medium composed of half concentration of Murashige and Skoog's salts and 2% sucrose. A two-stage cell suspension culture was developed for enhanced accumulation of L-DOPA. In the first stage, the culture system was composed of MSI medium without CaCl, which was suitable for cell growth and in the second stage MSI medium containing 42.5 mg.l−1 KH2PO4 and 4% sucrose favoured L-DOPA production. A discernible higher production of L-DOPA was obtained in this two-stage cell suspension culture in comparison to single stage culture.Keywords
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