Routine Assay of 11-Deoxy-17-Ketosteroids in Human Urinary Gas-Liquid Chromatography With a Standardized Column
- 1 September 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 48 (3) , 295-300
- https://doi.org/10.1093/ajcp/48.3.295
Abstract
A routine analytical procedure is described for the analysis of urinary 11-deoxy-17-ketosteroids using gas-liquid chromatography. The procedure involved the stepwise hydrolysis of steroid conjugates with [beta]-glucuronidase, and solvolysis with sulfuric acid followed by chromatography on silicic acid. The purified extracts were then subjected to gas-liquid chromatography on a 2 component (SE30-neopentylglycolsuccinate) highly reproducible column in the form of their trimethylsilyl ether (TMSi) derivatives. Over a period of 2 years, approximately 320 samples were analyzed by this technic. The mean urinary excretion patterns were determined by androsterone (A), etiocholanolone (E), and dehydroepiandrosterone (DHA) in various age groups of about 100 normal female subjects. In the postmenopausal group, a significant fall in A and E excretion levels was noted in contrast to that of DHA, which was unaffected. In pregnancy, a higher average level of DHA was excreted in comparison with that of nonpregnant females, whereas A and E did not exhibit this tendency.This publication has 1 reference indexed in Scilit:
- Microquantitative separation of isomeric dimethyltocols by gas-liquid chromatographyArchives of Biochemistry and Biophysics, 1966