Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF‐2
- 8 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 148 (2) , 214-220
- https://doi.org/10.1016/0014-5793(82)80810-7
Abstract
Ternary complex formation was studied in reticulocyte lysate supernatants and using rat liver eukaryotic initiation factor-2 (eIF-2) preparations. Haem-deficiency reduced the rate of formation of ternary (Met-tRNAf · GTP · eIF-2) complexes by the eIF-2 in reticulocyte supernatants, the reduction being more marked when complex formation was assayed in the absence of GTP-regenerating capacity. Pretreatment with the haem-controlled repressor (HCR) reduced the rate of ternary complex formation by crude (liver) eIF-2. In contrast, complex formation by an almost homogeneous eIF-2 preparation was unaffected by HCR: sensitivity to HCR was however restored by a factor which catalyses exchange of guanine nucleotides bound to eIF-2.Keywords
This publication has 34 references indexed in Scilit:
- Purification and phosphorylation of initiation factor eIF‐2 from rabbit skeletal muscleFEBS Letters, 1982
- Phosphorylation inhibits guanine nucleotide exchange on eukaryotic initiation factor 2Nature, 1982
- Regulation of Protein Synthesis in Rabbit Reticulocyte Lysates. Phosphorylation of eIF-2 Does Not Inhibit Its Capacity to RecycleEuropean Journal of Biochemistry, 1980
- Control of the initiation of protein synthesis in mammalian cellsFEBS Letters, 1980
- Stimulation of initiation factor eIF-2 by a rat liver protein with GDPase activityBiochemical and Biophysical Research Communications, 1980
- Purification and Characterization of a Protein Factor that Reverses the Inhibition of Protein Synthesis by the Heme‐Regulated Translational Inhibitor in Rabbit Reticulocyte LysatesEuropean Journal of Biochemistry, 1979
- Inhibition of cell-free protein synthesis by pppA2′ p5′ A2′ p5′ A: a novel oligonucleotide synthesized by interferon-treated L cell extractsCell, 1978
- Initiation of mammalian protein synthesisJournal of Molecular Biology, 1977
- Phosphorylation of initiation factor eIF-2 and the control of reticulocyte protein synthesisCell, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970