Characteristics of Batch Suspension Cultures of Preconditioned Coleus blumei Cells: Sucrose Effect

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Abstract
Batch suspension cultures of Coleus blumei cells preconditioned with 0.1% dimethyl sulfoxide (DMSO) maintained viability at above 85 % during a 14‐day culturing period for initial sucrose concentrations of 30–70 g/L. At 60 g/L sucrose, cells grew rapidly with a doubling time of 10.7 h, and dry cell mass reached a maximum of 15.6 g/L. Broad optima of cell yield (0.46–0.48 g of cell/g of sucrose), rosmarinic acid (RA) yield (3.1–3.5 g of RA/100 g of sucrose), and rosmarinic acid production (1.0–1.1 g of RA/L) were observed at 40–60 g/L sucrose concentration. Sucrose in the medium was hydrolyzed to glucose and fructose by extracellular and/or cell‐bound invertase, and glucose was taken up by the cells at a higher rate than fructose. Even at sucrose concentrations of 40–70 g/L, sucrose consumption over 14 days was limited to 33 g/L.