Fluorescent antibody technique as a means of localizing Ceratocystis ulmi toxins in elm

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Abstract
An aggressive strain of Ceratocystis ulmi was grown in a defined medium for the production of phytotoxins. Antisera to two distinct toxins, concanavalin A binding glycopeptides and cerato-ulmin, were prepared and used as probes for the detection and localization of the toxins in toxin-treated cuttings of elm. The toxins caused wilting of elm cuttings and were detected primarily on the pit membranes of bordered pits.