Mechanisms for radiation damage in DNA constituents and DNA. Reactions of the N1-substituted thymine π-cation radicals
- 1 January 1977
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Faraday Discussions of the Chemical Society
- Vol. 63, 255-263
- https://doi.org/10.1039/dc9776300255
Abstract
Reactions of the N1-substituted thymine π-cation radicals were investigated by e.s.r. in several aqueous glasses. In 8 mol dm–3 NaOD (NaOH) spectra suggestive of OD–(OH–) addition to position 6 in the π-cations of 1-methylthymine, and thymidine were found immediately after u.v. photolysis at 77 K. Production of the same radicals by electron attachment to 1-methyl-5-bromo-6-hydroxythymine and 5-bromo-6-hydroxythymidine in 8 mol dm–3 NaOD confirms the OD– addition mechanism. Results found for these brominated compounds in 12 mol dm–3 LiCl(D2O) after electron attachment show that the a6(H) splitting was sensitive to changes in substituents at position 1 as well as changes in environment. This variation in splitting is shown to be accounted for by small conformational changes in the radicals. In 8 mol dm–3 NaClO4π-cations of the substituted thymines gave evidence for both deprotonation and OD– addition.Keywords
This publication has 0 references indexed in Scilit: