IMMUNOREACTIVITY AND BIODISTRIBUTION OF INDIUM-111-LABELED MONOCLONAL-ANTIBODY TO A HUMAN HIGH MOLECULAR WEIGHT-MELANOMA ASSOCIATED ANTIGEN

  • 1 January 1985
    • journal article
    • research article
    • Vol. 26  (5) , 488-492
Abstract
The anti-human, high MW-melanoma associated antigen (HMW-MAA) MoAb 225.28S was chelated with 111In and then tested for its in vitro reactivity with cultured human melanoma cells and for its biodistribution in human melanoma bearing nude mice. In vitro studies showed that the radiolabeled antibody reacted specifcally with cultured melanoma cells. Binding of DTPA [diethylenetriaminepentaacetic acid] to the monoclonal antibody reduced its titer with cultured melanoma cells from 1:1024 to 1:512. Further labeling of the DTPA-antibody conjugate with 111In caused an additional reduction of its titer to 1:128. Injection of the radiolabeled monoclonal antibody into nude mice resulted in the accumulation of significantly (P < 0.001) higher radioactivity in melanoma tissue than in nude mice injected with either [111In]chloride or 111In-labeled antibody to human acid phosphatase. The specificity of the distribution of the radiolabeled antibody in nude mice also was indicated by its poor localization in lesions other than melanoma (e.g., human prostate carcinoma and chronic abscess). The localization of antibody in nude mice also was indicated by its poor localization in lesions other than melanoma (e.g., human prostate carcinoma and crhonic abscess). Th localization of antibody in results indicate that 111In-labeled monoclonal antibodies to human tumor associated antigens may be useful for localizing malignant lesions. There is a need to improve labeling and/or purification of antibody in order to decrease renal and hepatic activity.