Electrochemical detection of peptides
- 1 December 1989
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 61 (23) , 2664-2668
- https://doi.org/10.1021/ac00198a015
Abstract
A general method of wide applicability for the determination of peptides is described. Peptides longer than dipeptides react in the classical biuret reaction with Cu(II) to yield electroactive Cu(II)-peptide complexes that can be oxidized to the corresponding Cu(III) complexes. This allows the sensitive electrochemical detection of peptides following their separation by reversed-phase lipid chromatography. The reaction chemistry, which is reversible, allows for the determination of peptides that lack an electroactive group or a primary amine. Selectivity for a model peptide is 103-104 over nonelectroactive amino acids.This publication has 10 references indexed in Scilit:
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