Transient expression of foreign genes in lymphoid cells is enhanced by phorbol ester.
Open Access
- 1 July 1987
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 7 (7) , 2610-2613
- https://doi.org/10.1128/mcb.7.7.2610
Abstract
In lymphoid cells and an erythroleukemia cell line, phorbol myristate acetate significantly enhanced (up to 90-fold) expression of chloramphenicol acetyltransferase driven by four unrelated viral transcriptional units. The results suggest that phorbol myristate acetate acts via a general mechanism to enhance gene expression in these cells and provides a convenient means for increasing transient expression of foreign DNA.This publication has 17 references indexed in Scilit:
- cis-Acting transcriptional regulatory sequences in the gibbon ape leukemia virus (GALV) long terminal repeatVirology, 1987
- Two Different cis -Active Elements Transfer the Transcriptional Effects of Both EGF and Phorbol EstersScience, 1986
- Phorbol ester induces the transcriptional stimulatory activity of the SV40 enhancerNature, 1986
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- Trans -Acting Transcriptional Activation of the Long Terminal Repeat of Human T Lymphotropic Viruses in Infected CellsScience, 1984
- Immunoglobulin gene transcription is activated by downstream sequence elementsCell, 1983
- The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.Proceedings of the National Academy of Sciences, 1982
- Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.Proceedings of the National Academy of Sciences, 1982
- Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.The Journal of Experimental Medicine, 1980
- T‐Cell Growth FactorImmunological Reviews, 1980