Purification of NADH-Nitrate Reductase by Affinity Chromatography
- 1 December 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 56 (6) , 853-855
- https://doi.org/10.1104/pp.56.6.853
Abstract
Assimilatory nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of μmolar concentrations of NADH.Keywords
This publication has 6 references indexed in Scilit:
- Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.Proceedings of the National Academy of Sciences, 1975
- Rapid purification of lactate dehydrogenase from rat liver and hepatoma: A new approachArchives of Biochemistry and Biophysics, 1974
- Activation of nitrate reductase by oxidationBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
- Properties of a nitrate reductase of ChlorellaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
- Wechselwirkungen der Hefe‐Phosphofructokinase mit Dextranblau 2000European Journal of Biochemistry, 1971
- Further Purification and Properties of Neurospora Nitrate ReductaseJournal of Biological Chemistry, 1969