[An investigation of non-specific reactions in measurement of plasma (1-->3)-beta-D-glucan].

Open Access
Publisher Website
Google Scholar
Abstract
We investigated the detection of non-specific reaction in measurement of plasma (1-3)-β-Dglucan (β-glucan) by alkaline treatment, chromogenic automated kinetic assay (alkaline-kinetic assay) and dilution and heating method, chromogenic endpoint assay (dilution heating endpoint assay).In this study, we reexamined the values of β-glucan by both methods with and without 4-amidinophenyl benzoate hydrochloride (APB) as protease inhibitor that blocks Limulus reaction inthe 142 serum samples from 142 patients who had been treated and measured β-glucan in Kawasakimedical school hospital between January 1999 and May 1999. Non-specific reactions were judged bythe calculated value under APB additive condition. The non-specific reactions were found in 135 oftotal 142 samples (95.1%) in the alkaline-kinetic assay while no non-specific reactions were recognizedin dilution heating endpoint assay. The alkaline-kinetic assay has been used widely and been evaluatedit's usefulness because of good sensitivity. However, we found very high frequency of nonspecificreaction in this method. Further studies are needed to define the reasons of non-specific reaction.On the other hand, although non-specific reactions were not detected in dilution heating endpointassay, it's clinical utilities should be evaluated in future clinical studies.

This publication has 0 references indexed in Scilit: