TUMOR AND LYMPHOID-CELL LINES FROM A PATIENT WITH CARCINOMA OF COLON FOR A CYTOTOXICITY MODEL

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (5) , 1345-1355
Abstract
Three tumor cell lines (COLO 201, COLO 205 and COLO 206) were established from ascites fluid obtained from a male patient with adenocarcinoma of the colon. Two lymphoid lines (COLO 197 and COLO 200) were established from the same patient, with 1 line from the original biopsy and 1 from peripheral blood. Characterization of the tumor cell lines revealed 4 cell types that differ from most other colon cell lines. Chromosome markers were identical in COLO 201 and COLO 205. A long-arm isochromosome 5 observed in COLO 201 and COLO 205 was absent in COLO 206. Statistical analysis of autosomal polysomy revealed that these cell lines were stable and indicated that there may be a cytogenetic basis for the 3 predominant types of cell morphology. The lymphoid cell line derived from the peripheral blood had a normal male karyotype. The lymphoid cell line derived from a biopsy specimen had a mode of 46 and a deleted chromosome 7 marker. Both lymphoid cell lines had B[bone marrow-derived]-cell characteristics. These autochthonous cell lines were used for immunological studies in cytotoxicity assays and immunoglobulin characterization.