Involvement of protein kinase A and casein kinase II in the in vivo protein kinase activities in prophase arrested Xenopus oocytes
Open Access
- 1 June 1989
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 9 (3) , 351-358
- https://doi.org/10.1007/bf01114688
Abstract
In vivo β casein phosphorylation was analysed in Xenopus full-grown oocytes arrested in the prophase of the meiotic cell division. The phosphorylation was inhibited by the protein kinase inhibitor (PKI) and also by heparin (3 μg/ml; final concentration). β casein phosphorylation was increased by spermine (2 mM). Therefore, protein kinase A and casein kinase II are both active in vivo in full-grown oocytes and may be involved in the prophase arrest of meiotic cell division.This publication has 30 references indexed in Scilit:
- The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosisCell, 1988
- PROTEIN SERINE/THREONINE KINASESAnnual Review of Biochemistry, 1987
- A possible role for Mg2+ ions in the induction of meiotic maturation of Xenopus oocyteCell Differentiation, 1986
- Progesterone and cAMP-dependent protein kinase regulate in vivo the level of phosphorylation of two proteins (Mr 20,000 and Mr 32,000) in Xenopus oocytesDevelopmental Biology, 1986
- A potent synthetic peptide inhibitor of the cAMP-dependent protein kinase.Journal of Biological Chemistry, 1986
- ATP‐γ‐S (adenosine 5′‐0(3‐thiotriphosphate)) blocks progesterone‐induced maturation of the Xenopus oocyteJournal of Experimental Zoology, 1984
- Early increase of a 105,000-dalton phosphoprotein during meiotic maturation of Xenopus laevis oocyteBiochimie, 1983
- In vivo actions of protein phosphatase inhibitor‐2 inXenopusoocytesFEBS Letters, 1982
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animalsJournal of Morphology, 1972