Restriction Endonuclease Analyses of Two Porcine Mycoplasma Deoxyribonucleic Acids: Sequence-Specific Methylation in the Mycoplasma hyopneumoniae Genome
- 1 January 1984
- journal article
- research article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 34 (1) , 16-20
- https://doi.org/10.1099/00207713-34-1-16
Abstract
The DNA of 2 porcine mycoplasmas, M. hyopneumoniae and M. flocculare, were distinguished readily with restriction enzymes. Enzymes that recognized guanine-plus-cytosine-rich sequences, such as SmaI and BamHI, were particularly useful since they generated relatively small numbers of DNA fragments which could be resolved by gel electrophoresis. The adenine nucleotide in the GATC sequence of M. hyopneumoniae is methylated, whereas that in M. flocculare is not.This publication has 11 references indexed in Scilit:
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