The Relationship of Granulocyte ATP to Chemotactic Response During Storage

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Abstract
During storage of [human] granulocytes, bactericidal activity declined more slowly than did chemotactic response (CTR). Bacterial killing involved increased activity of the hexose monophosphate shunt, O2 utilization and generation of toxic O2 products. Chemotaxis was probably a contractile process involving myosin and actin filaments and possibly ATP as a source of energy. Maintainance of ATP may be important in granulocyte preservation. During storage at 1-6.degree. C of granulocytes collected by continuous and intermittent flow centrifuge leukapheresis, CTR and ATP decreased approximately 33%. Decreases in CTR and ATP were 12 and 10%, respectively, when cells were stord at 20-24.degree. C. Further decreases in CTR and ATP occurred between 24 and 48 h of storage, although levels were higher in cells stored at 20-24.degree. C compared with those stored at 1 to 6.degree. C. When the results from all storage conditions were combined, the overall correlation coefficient between CTR and ATP was 0.71 (P < 0.05). Although ATP is probably not the only important variable in granulocyte preservation, granulocytes may resemble red blood cells in that a minimal ATP level may be necessary for adequate function.