Comparison of Media Proteins from Ovariectomized Rat Uteri Following Estrogen Treatment

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Abstract
Ovariectomized rats were treated with estradiol for 3 days after which their uteri were incubated in vitro and radioactive media proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Media were also chromatographed on G-25 Sephadex Blue Sepharose columns to isolate subsets of proteins. Two proteins are consitently increased following estrogen treatment. These proteins have MW of 104,000 and 65,000. Neither protein binds to Blue Sepharose to a great extent. The use of the protein synthesis inhibitors, emetine and actinomycin D, demonstrates that the proteins are synthesized de novo. These 2 proteins may serve as markers for genomic response to estradiol in the rat uterus.