Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: a new method.
Open Access
- 1 November 1993
- journal article
- abstracts
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 41 (11) , 1635-1639
- https://doi.org/10.1177/41.11.8409371
Abstract
We combined immunocytochemistry and enzyme cytochemistry to localize two different proteins on the same ultrathin cryosection. In this method the immunocytochemical localization is visualized with colloidal gold probes and the enzyme cytochemical detection is achieved with cerium as the capture agent. The immunocytochemistry is conducted first so that any potential adverse effects of the enzyme cytochemical procedure will not alter the antibody binding properties of the cryosections.Keywords
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