The Intradermal Test in the Diagnosis of Schistosomiasis mansoni. IX. Skin Response to a Purified Fraction Isolated from Cercarial Extracts
- 1 October 1965
- journal article
- research article
- Published by JSTOR in Journal of Parasitology
- Vol. 51 (5) , 753-+
- https://doi.org/10.2307/3276151
Abstract
Butanol powder from lyophilized cercariae (Schistosoma mansoni) was extracted either with 0.9 NaCl or 0.1 [image] borate buffer pH 8.0, precipitated at pH 4.7 and supernatant (total extract) fractionated by column chromatography (DEAE-Sephadex). The fractions obtained were assayed for protein and polysaccharide and skin tested in patients with active schistosomiasis mansoni. Several fractions were found to elicit a skin response. One of them (fraction D, in which no polysaccharide was detected) was at least five times more active than the total extract and contained only 20% of the absoring material at 280 mu.This publication has 5 references indexed in Scilit:
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