An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis
- 19 August 2007
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 14 (9) , 875-877
- https://doi.org/10.1038/nsmb1288
Abstract
Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication.Keywords
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