Regulation of tumour necrosis factor α mRNA stability by the mitogen‐activated protein kinase p38 signalling cascade
Open Access
- 9 October 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 483 (1) , 57-61
- https://doi.org/10.1016/s0014-5793(00)02084-6
Abstract
The translation of tumour necrosis factor α (TNFα) mRNA is regulated by the stress‐activated protein kinase p38, which also controls the stability of several pro‐inflammatory mRNAs. The regulation of TNFα gene expression in a mouse macrophage cell line RAW264.7 was re‐examined using an inhibitor of stress‐activated protein kinases. Stimulation of these cells with bacterial lipopolysaccharide resulted in stabilisation of TNFα mRNA, which was reversed by specific inhibition of p38. An adenosine/uridine‐rich element from the TNFα 3′ untranslated region conferred p38‐sensitive decay in a tetracycline‐regulated mRNA stability assay. Therefore the p38 pathway also controls TNFα mRNA turnover.Keywords
This publication has 27 references indexed in Scilit:
- mRNA polyadenylation and its coupling to other RNA processing reactions and to transcriptionCurrent Opinion in Cell Biology, 1999
- Impaired On/Off Regulation of TNF Biosynthesis in Mice Lacking TNF AU-Rich ElementsImmunity, 1999
- Transcriptional Pulsing Approaches for Analysis of mRNA Turnover in Mammalian CellsMethods, 1999
- Feedback Inhibition of Macrophage Tumor Necrosis Factor-α Production by TristetraprolinScience, 1998
- The Role of 3′ Poly(A) Tail Metabolism in Tumor Necrosis Factor-α RegulationJournal of Biological Chemistry, 1997
- Mitogen and stress response pathways: MAP kinase cascades and phosphatase regulation in mammals and yeastCurrent Opinion in Cell Biology, 1995
- A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteinsPublished by Elsevier ,1994
- Interleukin-1 activates a novel protein kinase cascade that results in the phosphorylation of hsp27Cell, 1994
- Pyridinyl imidazoles inhibit IL-1 and TNF production at the protein levelInflammation Research, 1993
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986