IFN-γ increases cathepsin H mRNA levels in mouse macrophages

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Abstract
Expression of major histocompatibility complex (MHC) class II molecules and ability to present anti gen to T lymphocytes is acquired upon activation of the macrophage by intcrfcron-γ (IFN-γ). Little information is available concerning immune regulation of protease gene expression in mouse macrophages. We have isolated a eDNA clone for cathepsin H, a lysosomal cysteine pro teinase from a cDNA subtraction library of mouse macro phage genes induced by IFN-γ, and have characterized its expression. The level of cathepsin H mRNA increased in mouse peritoneal macrophages following addition of IFN-γ. Cathepsin H mRNA levels began to increase 8 h after the addition of IFN-γ and was maximal at 24–48 h. This increase was concordant in time with appearance of MHC class II Eβ mRNA and Ia invariant chain mRNA. The increase in cathepsin H mRNA levels by IFN-γ was dose dependent. Cycloheximide treatment of peritoneal macrophages inhibited the increase in cathepsin H mRNA levels induced by IFN-γ, suggesting that the in crease in cathepsin mRNA levels requires de novo protein synthesis. Lipopolysaccharide and cytokines interleukin-2 (IL-2), IL-4, IL-10, and tumor necrosis factor α were found to have no effect on cathepsin H mRNA levels in mouse peritoneal macrophages. J. Leukoc. Biol. 57: 663–669; 1995.

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