Detection of Lactoferrin and Myeloperoxidase Release from Single Neutrophils by a Protein A Plaque Assay

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Abstract
A haemolytic plaque assay was adopted to detect release of lactoferrin and mycloperoxidase (MPO) from single neutrophils. Target erythrocytes coated with protein A were bound as a monolayer by poly‐l‐lysine to the surface of plastic dish. Secreted lactoferrin and MPO induced plaque formation dependent on the reaction with complement and specific antiserum producing lysis of the protein‐A‐coated sheep red blood cells Lactoferrin was found to be released spontaneously from a fraction of neutrophils while MPO was released only after phagocytosis, reflecting different mechanisms for degranulation of MPO‐containing azurophil and lactoferrin‐containing specific granules.