Persistence of herpes simplex virus genes in cells of neuronal origin

Publisher Website
Google Scholar
Abstract
The growth characteristics of the KOS strain of herpes simplex virus type 1 (HSV-1) in cell lines of nervous tissue origin were examined to develop a tissue culture system mimicking the in vivo state of HSV-1 latency. The B103 rat brain neuroma cell line is nonpermissive for growth of the KOS strain. This report shows that this nonpermissiveness is a temperature- and multiplicity-dependent phenomenon, with minimum virus yields at an elevated temperature and a low multiplicity of infection. Under these conditions, B103 cells survived infection with active wild-type or mutant HSV-1; similarly treated Vero [African green monkey kidney] cells were killed. Six independent cultures of B103 cells surviving HSV-1 infection were established. The surviving cells ceased production of any HSV-1 virus by 14 days postinfection and resumed growth and division at rates comparable to those of uninfected B103 cells. Survivor cells continued to express HSV-1 specific antigens as detected by indirect immunofluorescence and by surface iodination followed by immunoprecipitation and polyacrylamide gel electrophoresis. The survivor cells did not express all of the surface proteins seen on productively infected B103 cells and they were not susceptible to complement-mediated immune cytolysis with anti-HSV-1 antiserum. Thus at least a portion of the HSV-1 genome is harbored in these survivor cells.