The FLP protein contacts both major and minor grooves of its recognition target sequence
Open Access
- 1 January 1992
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 20 (22) , 5927-5935
- https://doi.org/10.1093/nar/20.22.5927
Abstract
The FLP protein of the 2µm plasmid of Saccharomyces cerevisiae promotes conservative site-specific recombination between DNA sequences that contain the FLP recognition target (FRT). FLP binds to each of the three 13 base pair symmetry elements in the FRT site in a site-specific manner. We have probed both major and minor groove contacts of FLP using dimethyl sulphate, monoacetyl-4-hydroxyaminoquinoline 1-oxide and potassium permanganate and find that the protein displays extensive interactions with residues of both the major and minor grooves of 10 base pairs of each symmetry element. We find no evidence that the FRT site assumes a single-stranded conformation upon FLP binding.Keywords
This publication has 20 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- FLP protein of 2 μ circle plasmid of yeast induces multiple bends in the FLP recognition target siteJournal of Molecular Biology, 1990
- The N2-guanine adduct but not the C8-guanine of N6-adenine adducts formed by 4-nitroquinoline 1-oxide blocks the 3'-5' exonuclease action of T4 DNA polymeraseBiochemistry, 1990
- Missing contact probing of DNA-protein interactions.Proceedings of the National Academy of Sciences, 1987
- Isolation of intermediates in the binding of the FLP recombinase of the yeast plasmid 2-micron circle to its target sequenceJournal of Molecular Biology, 1987
- N2-GUANYL AND N6-ADENYL ARYLATION OF CHICKEN ERYTHROCYTE DNA BY THE ULTIMATE CARCINOGEN OF 4-NITROQUINOLINE 1-OXIDE1986
- The FLP recombinase of the 2μ circle DNA of yeast: Interaction with its target sequencesCell, 1985
- ADDUCTS FROM INVIVO ACTION OF THE CARCINOGEN 4-HYDROXYAMINOQUINOLINE 1-OXIDE IN RATS AND FROM INVITRO REACTION OF 4-ACETOXYAMINOQUINOLINE 1-OXIDE WITH DNA AND POLYNUCLEOTIDES1985
- Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.Proceedings of the National Academy of Sciences, 1980
- The selective degradation of pyrimidines in nucleic acids by permanganate oxidationBiochemical and Biophysical Research Communications, 1967