BIO-ASSAY OF CALORIGEN1C SUBSTANCES USING TADPOLES OF XENOPUS LAEVIS1

Publisher Website
Google Scholar
Abstract
A supplementary method for the bio-assay of thyroidal activity using the appearance of the fore limb buds of tadpoles (X. laevis) is descr. The significant attributes of this method are its speed and its sensitivity; it thus serves as a useful tool for the detection and assay of small amts. of calorigenic substances. Various calorigenic substances did not show activity proportional to their thyroxine content; natural thyroprotein was much more active than its thyroxine content would allow, whereas artificial iodoproteins were roughly proportional to their thyroxine content. Various standards of reference of activity were suggested for the assay of different calorigenic substances.