Ca++-Binding Properties of Canine Aortic Microsomes: Lack of Effect of c-AMP
- 1 January 1977
- journal article
- research article
- Published by S. Karger AG in Journal of Vascular Research
- Vol. 14 (2) , 91-104
- https://doi.org/10.1159/000158117
Abstract
Ca++ binding to a microsomal fraction from canine aorta has been studied and compared to Ca++ binding to canine cardiac microsomes. The aortic microsomes bound up to 60 nmoles Ca++/mg protein with a first-order binding rate constant of 0.025 sec–1. The binding rate in 5 sec was about half that of cardiac microsomes, 8 versus 15 nmoles/mg. The Ca++ binding to the aortic microsomes had a pH optimum of 7.4, was inhibited by monovalent cations and showed ATP preference when compared to other nucleotides. The binding was not stimulated by the presence of oxalate. c-AMP stimulated incorporation of 32P into the preparation, but had no effect on Ca++ binding.Keywords
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