Determination of serum retinol (vitamin A) by high-speed liquid chromatography.
Open Access
- 1 October 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 22 (10) , 1593-1595
- https://doi.org/10.1093/clinchem/22.10.1593
Abstract
We report a fast and simple high-speed liquid-chromatographic assay for serum retinol. Only 100 mul of serum is required. The lower detection limit is 50 mug/liter; linearity was demonstrated up to 1.50 mg/liter. On analyzing a serum pool eight times, a CV of 2.5% was obtained. Values by this method are compared with results obtained by a flurometric method [Clin. Chem. 16, 766(1970)].Keywords
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