NonO, a non-POU-domain-containing, octamer-binding protein, is the mammalian homolog of Drosophila nonAdiss.
- 1 September 1993
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 13 (9) , 5593-5603
- https://doi.org/10.1128/mcb.13.9.5593
Abstract
We have cloned the ubiquitous form of an octamer-binding, 60-kDa protein (NonO) that appears to be the mammalian equivalent of the Drosophila visual and courtship song behavior protein, no-on-transient A/dissonance (nonAdiss). A region unprecedently rich in aromatic amino acids containing two ribonuclear protein binding motifs is highly conserved between the two proteins. A ubiquitous form of NonO is present in all adult tissues, whereas lymphocytes and retina express unique forms of NonO mRNA. The ubiquitous form contains a potential helix-turn-helix motif followed by a highly charged region but differs from prototypic octamer-binding factors by lacking the POU DNA-binding domain. In addition to its conventional octamer duplex-binding, NonO binds single-stranded DNA and RNA at a site independent of the duplex site.Keywords
This publication has 49 references indexed in Scilit:
- Specific interaction of the first three zinc fingers of TFIIIA with the internal control region of the Xenopus 5 S RNA geneJournal of Molecular Biology, 1992
- Mutations in 5S DNA and 5S RNA have different effects on the binding of Xenopus transcription factor IIIABiochemistry, 1991
- Differential Expression of mRNA and Protein Encoding Retinal and Pineal S‐Antigen During the Light/Dark CycleJournal of Neurochemistry, 1990
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- Functional cooperativity between protein molecules bound at two distinct sequence elements of the immunoglobulin heavy-chain promoterNature, 1989
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988
- Cell-type specificity of iminunoglobulin gene expression is regulated by at least three DNA sequence elementsCell, 1985
- Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequenceCell, 1985
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970