Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing
Top Cited Papers
- 20 May 2005
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 2 (6) , 449-454
- https://doi.org/10.1038/nmeth761
Abstract
Introduction of exogenous DNA into mammalian cells represents a powerful approach for manipulating signal transduction. The available techniques, however, are limited by low transduction efficiency and low cell viability after transduction. Here we report a highly efficient molecular delivery technique, named nanotube spearing, based on the penetration of nickel-embedded nanotubes into cell membranes by magnetic field driving. DNA plasmids containing the enhanced green fluorescent protein (EGFP) sequence were immobilized onto the nanotubes, and subsequently speared into targeted cells. We have achieved an unprecedented high transduction efficiency in Bal17 B-lymphoma, ex vivo B cells and primary neurons with high viability after transduction. This technique may provide a powerful tool for highly efficient gene transfer into a variety of cells, especially the hard-to-transfect cells.Keywords
This publication has 24 references indexed in Scilit:
- Nanoscale Operation of a Living Cell Using an Atomic Force Microscope with a NanoneedleNano Letters, 2004
- Gene Therapy Progress and Prospects: Electroporation and other physical methodsGene Therapy, 2004
- Peritoneal and splenic B‐1 cells are separable by phenotypic, functional, and transcriptomic characteristicsEuropean Journal of Immunology, 2004
- Nanotube Molecular Transporters: Internalization of Carbon Nanotube−Protein Conjugates into Mammalian CellsJournal of the American Chemical Society, 2004
- Translocation of bioactive peptides across cell membranes by carbon nanotubesElectronic supplementary information (ESI) available: details of the synthesis and characterization, cell culture, TEM, epifluorescence and confocal microscopy images of CNTs 1, 2 and fluorescein. See http://www.rsc.org/suppdata/cc/b3/b311254c/Chemical Communications, 2003
- Multifunctional nanorods for gene deliveryNature Materials, 2003
- Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivoGene Therapy, 2002
- Growth and characterization of aligned carbon nanotubes from patterned nickel nanodots and uniform thin filmsJournal of Materials Research, 2001
- Synthesis of Large Arrays of Well-Aligned Carbon Nanotubes on GlassScience, 1998
- Tissue Culture and Scanning Electron Microscopy of Breast Carcinoma ‘Cell Line MCF-7’ from Pleural EffusionCells Tissues Organs, 1981