Phosphorylation of complement factor C3 in vivo
- 1 August 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 261 (3) , 1051-1054
- https://doi.org/10.1042/bj2611051
Abstract
Complement factor C3, the central protein of the complement system, was found to be phosphorylated both in EDTA- and heparin-anticoagulated whole blood and in coagulating blood. Complement S protein (vitronectin) was also found to be phosphorylated under these conditions. Further, purified C3 was found to be a phosphoprotein in vivo, containing 0.15 mol of alkali-labile phosphate/mol of protein. The ATP concentration in plasma was measured and found to be about 2 microM.This publication has 18 references indexed in Scilit:
- Vitronectin is phosphorylated by a cAMP-dependent protein kinase released by activation of human platelets with thrombinBiochemical and Biophysical Research Communications, 1988
- In vitro phosphorylation of serum albumin by two protein kinases: A potential pitfall in protein phosphorylation reactionsAnalytical Biochemistry, 1986
- Ecto-protein kinase activity on the external surface of neural cellsNature, 1986
- Identification, purification, and partial characterization of plasma protein kinasesArchives of Biochemistry and Biophysics, 1985
- Effect of sulfhydryl-disulfide state on protein phosphorylation: Phosphorylation of bovine serum albuminArchives of Biochemistry and Biophysics, 1985
- The location of a second in vivo phosphorylation site in the Aα-chain of human fibrinogenThrombosis Research, 1984
- A protein kinase system from platelet rich plasmaBiochemical and Biophysical Research Communications, 1983
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Glucagon-induced phosphorylation of pyruvate kinase (type L) in rat liver slicesBiochemical and Biophysical Research Communications, 1977
- On the occurrence of phosphorus in fibrinogenBiochimica et Biophysica Acta, 1963