Rapid processing of primary embryonic tissues for chromosome banding pattern analysis
- 1 January 1972
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 11 (5) , 424-435
- https://doi.org/10.1159/000130208
Abstract
This is a report of several improved and interdependent chromosome procedures. First, a method is described for dissociating fetal tissues with trypsin-EDTA so that the resulting cell cultures will generally yield good chromosome preparations within two to five days. Then, a method is outlined for making these chromosome preparations in such a way that the majority are suitable for chromosome banding techniques. The essential part of this procedure is that cells are dried in a stream of air of controlled temperature and humidity emanating from a simple apparatus. Finally, a quick trypsin-EDTA-Giemsa method is described which allows well-defined chromosome bands to be obtained consistently in preparations from cultures of many solid tissues, or blood leukocytes, if these are processed according to the methods outlined.Keywords
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