New substrate for fluorometric determination of gamma-glutamyltransferase activity in serum.
Open Access
- 1 May 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (5) , 879-881
- https://doi.org/10.1093/clinchem/23.5.879
Abstract
We describe the preparation of a new substrate, N-gamma-L-glutamyl-5-aminoisophthalic acid dimethyl ester hydro-chloride, for the fluorometric determination of gamma-glutamyltransferase activity in serum by the "front-surface" technique. Details of the resulting method are provided. The final reaction mixture contains 4 mmol of the substrate per liter of tris(hydroxymethyl)aminomethane (100 mmol/liter) and glycylglycine (75 mmol/liter) at pH 8.2 (25 degrees C).This publication has 1 reference indexed in Scilit:
- Determination of γ-glutamyl transpeptidase activity in human serum and urineClinica Chimica Acta; International Journal of Clinical Chemistry, 1962