Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
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Open Access
- 28 January 2007
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 25 (2) , 221-231
- https://doi.org/10.1038/nbt1282
Abstract
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.Keywords
This publication has 47 references indexed in Scilit:
- Genomics of Protein Secretion and Hyphal Growth in AspergillusPublished by Springer Nature ,2006
- Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatusNature, 2005
- Genome sequencing and analysis of Aspergillus oryzaeNature, 2005
- Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzaeNature, 2005
- The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomesNucleic Acids Research, 2004
- Filamentous fungi as cell factories for heterologous protein productionTrends in Biotechnology, 2002
- Production of Organic Acids by FungiPublished by Springer Nature ,2002
- On the safety of Aspergillus niger - a reviewApplied Microbiology and Biotechnology, 2002
- Codon usage tabulated from international DNA sequence databases: status for the year 2000Nucleic Acids Research, 2000
- Genetic maps of eight linkage groups of Aspergillus niger based on mitotic mappingCurrent Genetics, 1993