ELECTRON MICROSCOPE STUDY OF RED CELL MEMBRANES AFTER EXPERIMENTAL INFECTION WITH THE VIRUS OF FOOT-AND-MOUTH DISEASE

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Abstract
An electron microscope study was made, by a replica-transfer method, of hemolyzed red cells from guinea pigs inoculated with Vallee O strain of the virus of foot-and-mouth disease. Between 24 and 72 hrs. after inoculation, within which interval generalization of the disease reached its height and the red cells were found infective for normal guinea pigs, high density masses with mean diam. of 246 mu. were found associated with practically all of the thousands of red cell ghosts examined; these were absent from normal control material. The number and the density of these masses tended to increase from 24-72 hrs. after inoculation. They were disposed either singly, at random, or in lines or rings. 92 hrs. after inoculation, coinciding with the decline of general symptoms of the disease and with disappearance of infectivity of the red cells, the masses were no longer found. Dense particles, 20-70 mu. in diam., were detected within these masses in material derived 42 hrs. after inoculation.