E. coli galactose-1-phosphate uridyl transferase: N-terminal and C-terminal sequences
- 31 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 23 (3) , 167-175
- https://doi.org/10.1007/bf00219455
Abstract
A modified procedure for the purification of E. coli galactose-1-phosphate uridyl transferase (E.C. 2.7.6.12) was developed which reproducibly gives pure enzyme. The purified enzyme was shown to be a dimeric protein with a subunit molecular weight of 41,000 and its amino acid composition and content of free sulfhydryl groups were determined. The N-terminal and C-terminal amino acid sequences were found to be NH2-thr-gln-phe-asn-pro-val-asp and -ser(val leu)-ala-COOH respectively. This N-terminal sequence allowed the identification of the start of the transferase gene in the DNA sequence determined by GRINDLEY. Furthermore it appears to define a nine base intercistronic region between the epimerase and transferase genes.This publication has 34 references indexed in Scilit:
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