Phospholipids Containing Photoactivable Groups in Studies of Biological Membranes *
- 1 June 1980
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 346 (1) , 165-198
- https://doi.org/10.1111/j.1749-6632.1980.tb22099.x
Abstract
Photoactivable carbene precursors, aryl diazirines and trifluorodiazopropionates, were incorporated synthetically into the omega-positions of fatty acids, which were used to synthesize phospholipids. Extensive intermolecular C--H insertion reactions were demonstrated by photolysis of liposomes prepared from the above phospholipids. Structural analysis of the cross-linked products showed that the predominant sites of cross-linking were in the expected positions within the bilayer. Studies on the topography of a number of membrane proteins using the above phospholipids were initiated. Cross-linking of the photoactivable phospholipids to membrane-embedded proteins, glycophorin A, cytochrome b5, and gramicidin A, was demonstrated.Keywords
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