Glutathione biosynthesis in the isolated perfused rat lung: utilization of extracellular glutathione

Publisher Website
Google Scholar
Abstract
The isolated perfused rat lung catalyzed the biosynthesis of GSH when the sulfur amino acids cysteine or N-acetylcysteine, but not methionine, were supplied in the perfusion medium. The lung also had the capacity to utilize extracellular GSH for this purpose. Replenishment of intracellular GSH in perfused lungs from diethylmaleate-treated rats was pronounced even at 25 μM GSH in the perfusion medium. The utilization of extracellular GSH is probably primarily through extracellular break-down and resynthesis rather than direct uptake as indicated by the inhibitory effect of the γ-glutamylcysteine synthetase inhibitor, buthionine sulfoximine and the γ-glutamyl transferase inhibitor, anthglutin. The results indicate that the lung in addition to the kidney may utilize circulating plasma GSH.