Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution
- 6 December 2001
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- No. 24,p. 2664-2665
- https://doi.org/10.1039/b109913m
Abstract
The combination of error prone PCR at high mutation rate and DNA shuffling can be used to invert the direction of enantioselectivity of a lipase-catalyzed hydrolytic kinetic resolution involving a chiral ester.Keywords
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