Methylation of 3′,4′ Di-O H C-Glycosyl-flavones in Silene
Open Access
- 1 June 1981
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 36 (5-6) , 486-487
- https://doi.org/10.1515/znc-1981-5-625
Abstract
In green parts of Silene plants of the genotype glR two methylated flavonoids were found: isoscoparin and isosco-parin 2″-O-rhamnoside. An enzyme has been demonstrated to catalyze the transfer of the methyl moiety of S-adenosyl methionine to iso-orientin and iso-orientin 2″-O-rhamnosi-de. Maximal activity takes place at pH 8.0 -8 .2 . Of the metal ions Mn2+, Mg2+, Ca2+, Co2+, Zn2+ and Hg2+, only Co2+ stimulated the reaction at conc. > 2 mM . For the methyla tion of isoorientin the Km values were 4 × 10-6 M for S-ade-nosyl methionine and 0.32 × 10-3 m for iso-orientin. When isoorientin 2″-O-rhamnoside was used as substrate the Km values were 5 × 10-6 m for S-adenosylmethionine and 7 × 10-6 M for iso-orientin 2″-O-rhamnoside.Keywords
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