Complete amino acid sequence of bovine colostrum low‐Mr cysteine proteinase inhibitor
- 1 July 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 186 (1) , 41-45
- https://doi.org/10.1016/0014-5793(85)81335-1
Abstract
The complete amino acid sequence of bovine colostrum cysteine proteinase inhibitor was determined by sequencing native inhibitor and peptides obtained by cyanogen bromide degradation, Achromobacter lysylendopeptidase digestion and partial acid hydrolysis of reduced and S-carboxymethylated protein. Achromobacter peptidase digestion was successfully used to isolate two disulfide-containing peptides. The inhibitor consists of 112 amino acids with an M r of 12787. Two disulfide bonds were established between Cys 66 and Cys 77 and between Cys 90 and Cys 110. A high degree of homology in the sequence was found between the colostrum inhibitor and human γ-trace, human salivary acidic protein and chicken egg-white cystatin.Keywords
This publication has 23 references indexed in Scilit:
- Isolation of a human cDNA for .alpha.2-thiol proteinase inhibitor and its identity with low molecular weight kininogenBiochemistry, 1984
- Amino acid sequence of rat epidermal thiol proteinase inhibitorBiochemical and Biophysical Research Communications, 1984
- The place of human γ-trace (cystatin C) amongst the cysteine proteinase inhibitorsBiochemical and Biophysical Research Communications, 1984
- Amino acid sequence of rat liver thiol proteinase inhibitorBiochemical and Biophysical Research Communications, 1983
- Isolation and immunological studies of high and low molecular weight cysteine proteinase inhibitors in bovine serumBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- Protein Inhibitors of Cysteine Proteinases. II. Primary Structure of Stefin, a Cytosolic Protein Inhibitor of Cysteine Proteinases from Human Polymorphonuclear GranulocytesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
- Protein Inhibitors of Cysteine Proteinases. III. Amino-Acid Sequence of Cystatin from Chicken Egg WhiteHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
- Low molecular weight inhibitors of cathepsins B, H and T in human serum, synovial fluid and CSFBiochemical and Biophysical Research Communications, 1982
- Existence of two kinds of thiol protease inhibitors in rat serumBiochemical and Biophysical Research Communications, 1982
- Thermostable Endogenous Inhibitors of Cathepsins B and HEuropean Journal of Biochemistry, 1979