Glycosphingolipids of the globo-series are associated with the monocytic lineage of human myeloid cells

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Abstract
Neutral glycosphingolipids (neutral GSL) of the human myeloid leukemia cell lines ML-2, ML-3, HL-60 and THP-1-0 were metabolically labeled with [3H]galactose and [3H]glucosamine, and analyzed by high-performance liquid chromatography. They were compared with unlabeled neutral GSL from purified human granulocytes and monocytes. Neutral GSL were identified by retention times and the structures were further confirmed by degradation with specific exoglycosidases. Two neutral GSL of the globo series, globotetraosylceramide and globotriaoslyceramide, were found in monocytes and the monoblastic leukemia line THP-1-0. The leukemia derived cell-lines, ML-3 and HL-60, representing successively earlier stages of myeloid differentiation, contained respectively less neutral GSL of the globo series and an increasing proportion of (neo)lacto neutral GSL. Granulocytes and the cell line ML-2 contained almost exclusively neutral GSL of the (neo)lacto series.