Independence between ionic transport and net water flux in frog gastric mucosa

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Abstract
The relationship be-tween the depression of ionic transport, induced by the use of sulfate solutions, and spontaneous water movement was investigated in isolated frog (Rana pipiens) gastric mucosa maintained in vitro. In 10 mucosae, maintained in chloride solutions, the following values were obtained: acid secretion 0. 57 [plus or minus] 0. 13 Eq/cm2hr.: short-circuit current 1. 18 [plus or minus] 0.12 Eq/cm2hr.; electrical potential difference -20 [plus or minus] 2 mv (as referred to the serosal surface), and net water flux 12 [plus or minus] 1 l/cm2hr. In the same mucosae, the following values were obtained when the chloride solutions were replaced by sulfate solutions: acid secretion 0.15 [plus or minus] 0. 04 Eq/cm2hr.; short-circuit current 0. 01 [plus or minus] 0. 04 Eq/cm2hr.; electrical potential difference 0 [plus or minus] 1 mv and net water flux 12 [plus or minus] 2 l/cm2hr. In chloride solutions the secretion had a mean chloride concentration of 98. 3 mEq/l and a mean HCl concentration of 47. 5 mEq/l with an osmolality 1. 3 times that of the chloride solutions. In sulfate solutions the secretion had a mean sulfate concentration of 0. 8 mEq/l and a mean H2SO4 concentration of 12. 5 mEq/l with an osmolality 0. 09 times that of the sulfate solutions. This suggests that the mechanisms responsible for the acid secretion and ionic accumulation in the secretory solutions are independent from those responsible for the net water flux observed in frog gastric mucosa, at least during the period in which they were maintained in sulfate solutions.

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