Tryptic Digestion of Native Small‐Intestinal Sucrase · Isomaltase Complex: Isolation of the Sucrase Subunit
- 1 April 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 52 (3) , 481-486
- https://doi.org/10.1111/j.1432-1033.1975.tb04017.x
Abstract
Limited tryptic digestion of native sucrase - isomaltase complex produced a more rapid destruction of isomaltase activity than sucrase activity. It was possible to isolate a partially fragmented sucrase subunits in high yields with a specific activity twice that of the native complex. Amino acid and carbohydrate analyses are reported and compared with the results obtained for sucrase - isomaltase complex and isomaltase subunit obtained by a different method.Keywords
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