A repressible female-specific lethal genetic system for making transgenic insect strains suitable for a sterile-release program
- 11 July 2000
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 97 (15) , 8229-8232
- https://doi.org/10.1073/pnas.140142697
Abstract
We have developed a tetracycline-repressible female-specific lethal genetic system in the vinegar fly Drosophila melanogaster. One component of the system is the tetracycline-controlled transactivator gene under the control of the fat body and female-specific transcription enhancer from the yolk protein 1 gene. The other component consists of the proapoptotic gene hid under the control of a tetracycline-responsive element. Males and females of a strain carrying both components are viable on medium supplemented with tetracycline, but only males survive on normal medium. A strain with such properties would be ideal for a sterile-insect release program, which is most effective when only males are released in the field.Keywords
This publication has 33 references indexed in Scilit:
- Insect Population Control Using a Dominant, Repressible, Lethal Genetic SystemScience, 2000
- Building the Better BugScientific American, 1998
- Gene Transfer into the Medfly, Ceratitis capitata , with a Drosophila hydei Transposable ElementScience, 1995
- Two independent cis-acting elements regulate the sex- and tissue-specific expression ofyp3inDrosophila melanogasterGenetics Research, 1995
- Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitataGenome, 1994
- Signalling by the sevenless protein tyrosine kinase is mimicked by Rasl activationNature, 1992
- Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinaseCell, 1991
- A tissue-specific transcription enhancer from the Drosophila yolk protein 1 geneCell, 1986
- Genetic Transformation of Drosophila with Transposable Element VectorsScience, 1982
- The Eradication of the Screw-Worm FlyScientific American, 1960