Preparation and characterization of neutralizing monoclonal antibodies to feline calicivirus.
- 1 January 1990
- journal article
- research article
- Published by Japanese Society of Veterinary Science in The Japanese Journal of Veterinary Science
- Vol. 52 (2) , 251-256
- https://doi.org/10.1292/jvms1939.52.251
Abstract
Fourteen neutralizing monoclonal antibodies (N-MoAbs) were prepared against the F4 strain of feline calicvirus (FCV), the prototype strain of FCV in Japan, and examined for their ability to neutralize FCV isolates. Neutralization-resistant variants of the F4 strain were selected under the presence of 4 individual N-MoAbs in cell culture systems and used in cross-neutralization tests and enzyme-linked immunosorbent assay with all of the 14 N-MoAbs. The results revealed the identification of at least two antigenic determinants on FCV F4: one being more broadly conserved among FCV isolates than the other. Usefulness of antigenic variants resistant to N-MoAbs for analysis of neutralization determinants on FCV was also demonstrated.This publication has 5 references indexed in Scilit:
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